SEC22B Background Information The S. cerevisiae protein Sec22p functions as a v-SNARE of transport vesicles and plays a role in both retrograde and anterograde vesicular transport between the Golgi and the endoplasmic reticulum (ER). There are three mammalian homologs to Sec22p, namely SEC22A, SEC22B and SEC22C. SEC22B (SEC22 vesicle trafficking protein homolog B, S. cerevisiae), also known as SEC22L1 (SEC22 vesicle-trafficking protein-like 1) or ERS-24, is a single pass type IV membrane protein that belongs to the synaptobrevin family. SEC22B contains one v-SNARE coiled-coil homology domain and one longin domain. Localizing to the ER-Golgi intermediate compartment and found on ER-derived vesicles, SEC22B functions as a v-SNARE and is required for ER-Golgi transport. The cognate t-SNARE found on pre-Golgi intermediate compartments is comprised of Syntaxin 5, Bet1 and GS27. In addition, SEC22B can be found in a SNARE complex with Syntaxin 18, Nip1 and USE1 (p31).
SEC22B (29-F7)
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SEC22B (29-F7): sc-101267. Western blot analysis of SEC22B expression in HeLa whole cell lysate.
SEC22B (29-F7): sc-101267. Immunofluorescence staining of paraformaldehyde-fixed HeLa cells showing nuclear and cytoplasmic localization.
SEC22B (29-F7): sc-101267. Western blot analysis of SEC22B expression in non-transfected 293T: sc-117752 (A), human SEC22B transfected 293T: sc-174592 (B) and HeLa (C) whole cell lysates.
