DBC-1 Background Information DBC-1 (deleted in breast cancer gene 1 protein), also known as p30 DBC protein, is one of the genes located within the region of chromosome 8 (8p21-8p23) that is homozygously deleted in some breast cancers. DBC-1 contains a nuclear localization signal, an N-terminal leucine zipper, an EF hand and a C-terminal coiled-coil region. DBC-1 is closely related to DIS but lacks the SAP domain. During death signaling mediated by TNFå, endogenous DBC-1 undergoes caspase-dependent processing to generate DBC-1 p120 and p66, both of which include the C-terminus of the protein. Both DBC-1 p120 and p66 relocate to the cytoplasm. Overexpression of the DBC-1 p120 form results in mitochondrial clustering and matrix condensation and increases the sensitivity of cells to TNFå-mediated apoptosis. In addition, DBC-1 directly interacts with unliganded ERå, stabilizing its expression and therefore collaborating to suppress apoptosis and promote hormone-independent cell growth.
DBC-1 (WQ7)
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DBC-1 (WQ7): sc-101228. Western blot analysis of DBC-1 expression in HeLa whole cell lysate.
DBC-1 (WQ7): sc-101228. Western blot analysis of DBC-1 expression in A549 whole cell lysate.
DBC-1 (WQ7): sc-101228. Immunofluorescence staining of paraformaldehyde-fixed HeLa cells showing cytoplasmic localization.
DBC-1 (WQ7): sc-101228. Western blot analysis of DBC-1 expression in non-transfected: sc-117752 (A) and human DBC-1 transfected: sc-116793 (B) 293T whole cell lysates.
DBC-1 (WQ7): sc-101228. Western blot analysis of DBC-1 expression in non-transfected: sc-117752 (A) and human DBC-1 transfected: sc-116793 (B) 293T whole cell lysates.
