TEF-3 Antibody (N-G2): sc-101184

TEF-3 Antibody (N-G2) is a mouse monoclonal IgG2a kappa light chain antibody that detects TEF-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-TEF-3 antibody (N-G2) is available as a non-conjugated format. TEF-3, also known as TEAD4 (TEA domain family member 4), RTEF1, EFTR-2, TEFR-1, TCF13L1, or hRTEF-1B, is a 427 amino acid protein that plays a crucial role in muscle development and regeneration. TEF-3 is primarily localized in the nucleus, where TEF-3 acts as a transcriptional regulator by binding to the M-CAT motif in promoters of muscle-specific genes, thus facilitating their expression. TEF-3′s importance lies in its ability to influence muscle differentiation and growth, making TEF-3 a key player in muscle-related diseases and conditions. Additionally, TEF-3 undergoes alternative splicing, resulting in multiple isoforms that may have distinct regulatory functions. This versatility in structure and function underscores TEF-3′s significance in cellular processes and potential as a therapeutic target in muscle disorders.

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TEF-3 Antibody (N-G2) References:

1. TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm.  |  Vassilev, A., et al. 2001. Genes Dev. 15: 1229-41. PMID: 11358867
2. Transcription enhancer factor-1-related factor-transgenic mice develop cardiac conduction defects associated with altered connexin phosphorylation.  |  Chen, HH., et al. 2004. Circulation. 110: 2980-7. PMID: 15520314
3. The 110kDa glutathione transferase of Yarrowia lipolytica is encoded by a homologue of the TEF3 gene from Saccharomyces cerevisiae: cloning, expression, and homology modeling of the recombinant protein.  |  McGoldrick, S., et al. 2005. Biochem Biophys Res Commun. 337: 1125-32. PMID: 16229819
4. Identification of novel alternatively spliced isoforms of RTEF-1 within human ocular vascular endothelial cells and murine retina.  |  Appukuttan, B., et al. 2007. Invest Ophthalmol Vis Sci. 48: 3775-82. PMID: 17652751
5. Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development.  |  Yagi, R., et al. 2007. Development. 134: 3827-36. PMID: 17913785
6. Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos.  |  Nishioka, N., et al. 2008. Mech Dev. 125: 270-83. PMID: 18083014
7. Transcription enhancer factor 3 (TEF3) mediates the expression of Down syndrome candidate region 1 isoform 1 (DSCR1-1L) in endothelial cells.  |  Liu, X., et al. 2008. J Biol Chem. 283: 34159-67. PMID: 18840614
8. Characterization of the transcriptional activation domains of human TEF3-1 (transcription enhancer factor 3 isoform 1).  |  Qiao, C., et al. 2015. Arch Biochem Biophys. 569: 54-61. PMID: 25687649
9. Nuclear localization of TEF3-1 promotes cell cycle progression and angiogenesis in cancer.  |  Teng, K., et al. 2016. Oncotarget. 7: 13827-41. PMID: 26885617
10. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation.  |  Hsu, DK., et al. 1996. J Biol Chem. 271: 13786-95. PMID: 8662936
11. A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain.  |  Jacquemin, P., et al. 1996. J Biol Chem. 271: 21775-85. PMID: 8702974
12. Cloning of human RTEF-1, a transcriptional enhancer factor-1-related gene preferentially expressed in skeletal muscle: evidence for an ancient multigene family.  |  Stewart, AF., et al. 1996. Genomics. 37: 68-76. PMID: 8921372

The preferred TEF-3 antibody is TEF-3 Antibody (B-5): sc-390578.