epitope mapping at the N-terminus of TLF of human origin
recommended for detection of TLF of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
TLF Background Information The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery (1). TBP and the various RNA polymerase subunits are assembled with unique TBP-associated factors (TAFs) into distinct complexes that act specifically with either RNA polymerase I (SL1/TIF-IB), RNA polymerase II (TFIID), or RNA polymerase III (TFIIIB) on cognate promoters (2,3). TLF (also called TBP-related factor 2 [TRF2]) activates a number of different genes, including the neurofibromatosis type 1 (NF1) gene. TLF is related in sequence and structure to TBP and the Drosophila TBP-related factor TRF1. TLF functions as gene-specific factor for RNA polymerase II-mediated transcription, but unlike TBP, TLF does not appear to be universal binding factor of other RNA polymerase complexes (4,5). TLF preferentially binds to and forms a stable complex with TFIIA (6). TFIIA is required as a core promoter selective factor for both basal and activated TFIID-mediated transcription as it enhances TBP/TFIID binding to DNA and alleviates TFIID repression that is mediated by negative cofactors (7,8).
