ME1 Background Information ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.
ME1 (99.1)
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ME1 (99.1): sc-100569. Western blot analysis of ME1 expression in non-transfected: sc-117752 (A) and human ME1 transfected: sc-172177 (B) 293T whole cell lysates.
ME1 (99.1): sc-100569. Western blot analysis of ME1 expression in Hep G2 whole cell lysate.
ME1 (99.1): sc-100569. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing cytoplasmic localization.
ME1 (99.1): sc-100569. Western blot analysis of ME1 expression in HeLa whole cell lysate.
