Welcome to Santa Cruz Biotechnology!
SCBT Home
Home » Chemicals » HRP and AP Substrates

HRP and AP Substrates

Introducing HOVERcruz™,

a unique system for rapid identification of chemicals of interest. Hover over product names in the table to see representative images for each product.

Live chat is now available! (click here)

Our live chat system is available to answer your product and ordering questions.

PRODUCT NAMECATALOG #DESCRIPTIONCITATIONSRANKING
3-Nitrotetrazolium blue chloridesc-209660An isomer of p-Nitroblue Tetrazolium Chloride (sc-296003) that has been used as an alkaline phosphatase substrate, superoxide radical scavenging activity detector, and in irradiation studies. CAS # 127615-60-5

3,3'-Diaminobenzidine tetrahydrochloride hydratesc-209686A water-soluble preparation of 3,3’-Diaminobenzidine. Used as a histological stain for detecting peroxidase activity. CAS # 868272-85-9

AEC Substrate Buffer, 10Xsc-36873For IHC staining procedures, a 10X buffer diluent for use with AEC, 50X (3-amino-9-ethylcarbazole) (sc-24979) composed of 0.5M acetate buffer in proprietary stabilizers, pH 5.0 ± 0.2 when diluted to 1X. CAS # 132-32-1 (solid)

AEC, 50Xsc-249793-amino-9-ethylcarbazole; for IHC staining procedures using HRP-based detection systems; provides red chromagen depositions. Recommended for use with AEC Substrate Buffer, 10X (sc-36873) and Crystal Mounting Medium (sc-24943) CAS # 132-32-1

15
BCIP-NBT Solutionsc-358798Pre-mixed, ready to use substrate for AP-based detection systems; gives a dark-blue oxidation product for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots.

BCIP/INT, 1Xsc-24980Ready to use substrate for alkaline phosphate (AP) based detection systems. Produces a yellow-brown color precipitate at the reaction sites, suitable for IHC, ICC and in situ hybridization procedures.

7
BCIP/NBT Stock Solution, 50Xsc-24981Stock substrate solution for AP-based detection systems; gives a dark-blue oxidation product for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots.

15
BCIP®/NBT-Blue Liquid Substrate System for Membranessc-291929A ready-to-use buffered alkaline phosphatase substrate for use in immunoblotting. Produces an insoluble dark blue-purple end product. Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

DAB, 50Xsc-249823-3′ diaminobenzidine tetrachloride (DAB); stock solution for HRP-based immunoblotting and immunohistochemical assays; sites of HRP activity appear as brown-orange deposits. CAS # 7411-49-6

23
Fast Red Tabletssc-24983A chromogen substrate for AP-based detection systems; recommended for use in fluorescent microscopy, immunoblotting, immunohistochemistry, in-situ hybridization and flow cytometric assays. CAS # 3165-93-3

4
Naphthol AS-MX phosphate disodium salt nonahydratesc-215540Used in histochemical detection of alkaline phosphatase.; Enzyme-incorporated erythrocyte ghosts. CAS # 36889-52-8

PNPP, Disodium Saltsc-3720Colorimetric alkaline phosphatase (AP) soluble substrate used in ELISA applications, produces a soluble end product that is yellow in color. Absorbs light at 405nm. Recommended for use with pNPP Substrate Buffer (sc-296099). CAS # 4264-83-9

11
PPHPsc-204856A substrate for the measurement of peroxidase enzymes and has been used to quantitate the peroxidase activity of COX-1 and COX-2.1,2,3 CAS # 87864-20-8

TMB Substrate Buffer, 1Xsc-286967TMB Substrate Solution is a single component, ready-to-use substrate for the enzyme horseradish peroxidase (HRP) used in ELISA detection systems. Develops a soluble blue reaction product that may be read at 655nm.

3,3′-Diaminobenzidinesc-2165673,3′-Diaminobenzidine is a peroxidase substrate and a reagent for spectrophotometric determination of selenium. DAB is used for the immunohistochemical staining of nucleic acids and proteins. CAS # 91-95-2