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HRP and AP Substrates

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PRODUCT NAMECATALOG #DESCRIPTIONCITATIONSRANKING
3-Nitrotetrazolium blue chloridesc-209660An isomer of p-Nitroblue Tetrazolium Chloride (sc-296003) that has been used as an alkaline phosphatase substrate, superoxide radical scavenging activity detector, and in irradiation studies. CAS # 127615-60-5

3,3'-Diaminobenzidine tetrahydrochloridesc-209686A water-soluble preparation of 3,3’-Diaminobenzidine. Used as a histological stain for detecting peroxidase activity. CAS # 868272-85-9

AEC Substrate Buffer, 10Xsc-36873For IHC staining procedures, a 10X buffer diluent for use with AEC, 50X (3-amino-9-ethylcarbazole) (sc-24979) composed of 0.5M acetate buffer in proprietary stabilizers, pH 5.0 ± 0.2 when diluted to 1X. CAS # 132-32-1 (solid)

AEC, 50Xsc-24979AEC, 50X (3-amino-9-ethylcarbazole); for IHC staining procedures using HRP-based detection systems; provides red chromagen depositions. CAS # 132-32-1

15
BCIP-NBT Solutionsc-358798Pre-mixed, ready to use substrate for AP-based detection systems; gives a dark-blue oxidation product for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots.

BCIP/INT, 1Xsc-24980BCIP/INT, 1X is ready to use substrate for alkaline phosphate (AP) based detection systems. Produces a yellow-brown color precipitate at the reaction sites, suitable for IHC, ICC and in situ hybridization procedures.

7
BCIP/NBT Stock Solution, 50Xsc-24981BCIP/NBT Stock Solution, 50X is substrate for AP-based detection systems; gives a dark-blue oxidation product for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots.

15
BCIP®/NBT-Blue Liquid Substrate System for Membranessc-291929A ready-to-use buffered alkaline phosphatase substrate for use in immunoblotting. Produces an insoluble dark blue-purple end product. Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

DAB, 50Xsc-249823-3′ diaminobenzidine tetrachloride (DAB); stock solution for HRP-based immunoblotting and immunohistochemical assays; sites of HRP activity appear as brown-orange deposits. CAS # 7411-49-6

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Fast Red Tabletssc-24983A chromogen substrate for AP-based detection systems; recommended for use in fluorescent microscopy, immunoblotting, immunohistochemistry, in-situ hybridization and flow cytometric assays. CAS # 3165-93-3

4
Naphthol AS-MX phosphate disodium salt nonahydratesc-215540Naphthol AS-MX phosphate disodium salt nonahydrate is used in histochemical detection of alkaline phosphatase; enzyme-incorporated erythrocyte ghosts. CAS # 36889-52-8

PNPP, Disodium Saltsc-3720Colorimetric alkaline phosphatase (AP) soluble substrate used in ELISA applications, produces a soluble end product that is yellow in color. Absorbs light at 405nm. Recommended for use with pNPP Substrate Buffer (sc-296099). CAS # 4264-83-9

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PPHPsc-204856A small molecule peroxide probe for peroxidase activity. Conversion to 5-phenyl-4-pentenyl alcohol by peroxidases can be traced with HPLC to assay activity. CAS # 87864-20-8

TMB Substrate Buffer, 1Xsc-286967TMB Substrate Solution is a single component, ready-to-use substrate for the enzyme horseradish peroxidase (HRP) used in ELISA detection systems. Develops a soluble blue reaction product that may be read at 655nm.

3,3′-Diaminobenzidinesc-2165673,3′-Diaminobenzidine is a peroxidase substrate and a reagent for spectrophotometric determination of selenium. DAB is used for the immunohistochemical staining of nucleic acids and proteins. CAS # 91-95-2