Santa Cruz Biotechnology, Inc. now offers over 141,049 specialty biochemicals under our ChemCruz™ brand for biomedical, life science, molecular biology and proteomics research. Biochemicals are necessary for a wide variety of chemical processes and intricate biological pathways which influence reproduction, disease and survival. Biochemicals have become dynamic tools in research for analyzing and understanding biological mechanisms and their components. We provide a comprehensive menu of standard laboratory chemicals for Western blot, immunostaining, RNAi studies and other essential laboratory experiments. Tris (sc-3715), NaCl (sc-203274), HEPES (sc-29097), NaOH (sc-203387) and Glycine (sc-29096) are among the most widely used standard laboratory chemicals required for conducting biological analysis. In addition to our wide range of standard laboratory chemicals, we now offer an extensive variety of substrates, enzymes, activators and inhibitors for cell signaling, apoptosis, cell cycle, cell adhesion, neurobiology and phosphorylation studies.

Santa Cruz Biotechnology supplies over 1,140 stains and dyes, such as DAPI (sc-3598), a commonly used fluorescent stain that preferentially binds adenine (A) and thymidine (T) rich DNA, chromosomes and nuclei. Coomassie Blue R-250 (sc-24972), also known as Brilliant Blue R-250, is a protein dye used in SDS-PAGE and for protein determination using the Bradford Method. As a membrane impermeable DNA intercalator, Propidium Iodide (sc-3541) is commonly used in flow cytometry for staining apoptotic cells and nuclei. Hematoxylin (sc-24973) is a blue-violet nuclear counter stain developed specifically for immunohistochemical applications. With reduced hematein and aluminum concentrations, Hematoxylin eliminates the danger of overstaining, will not stain glass or plastic containers or form surface or bottom precipitates.

Activating and inhibiting biochemicals are available for modifying biological responses. In cell culture, Hydroxyurea (sc-29061) inhibits DNA synthesis via deoxynucleotide blockage, which in turn induces apoptosis, while PMA (sc-3576) induces PKC phosphorylation and expression of E-Selectin. Calyculin A (sc-24000) is a potent non-phorbol type tumor promoter known to increase phosphorylation of PRC1 and Histone H3. Okadaic Acid (sc-3513) is a PP1 and PP2A phosphatase inhibitor, while Wortmannin (sc-3505) is a PI-3 kinase inhibitor that selectively inhibits PI-4 kinase and acts indirectly on p70 S6 kinase. Staurosporine (sc-3510) is a tyrosine kinase, topoisomerase II and PKC inhibitor that binds ATP binding domains. Ligands are signal triggering molecules that bind receptors, leading to alteration of receptor protein conformation. Investigating ligand-receptor interactions is vital for drug design, treatment of diseases and most importantly understanding signal transduction pathways such as apoptosis, cell cycle progression and hormone mechanism of action. Tamoxifen and its metabolite, (E)-4-Hydroxytamoxifen (sc-205662), are drugs that act as antagonists of the estrogen receptor that inhibit receptor activity.

With the rapid growth of RNAi studies, Santa Cruz Biotechnology's line of antibiotics and cell selection agents has progressively expanded. Puromycin dihydrochloride (sc-108071) is the recommended cell selection agent for RNAi experiments using Santa Cruz Biotechnology shRNA and Lentiviral Particle products which contain Puromycin resistance markers. As a protein synthesis inhibitor, Puromycin dihydrochloride causes premature chain termination, thereby inducing apoptosis in a variety of cells. Hygromycin B (sc-29067) is used for selection and maintenance of cells transfected with the Escherichia coli hygromycin resistance gene, whereas Geneticin (G418) Sulfate (sc-29065) is recommended for selection and maintenance of cells transfected with the neomycin (neo) resistance gene.