| |
Plasmid vectors are commonly used for the expression of coding regions of eukaryotic genes in E. coli. Such expression vectors often encode hybrid fusion proteins containing a eukaryotic specified protein fused on a tag protein. Antibodies specific for such fusion protein tags are useful for monitoring protein expression both in plaques and on Western immunoblots as well as for immunoaffinity purification of proteins expressed in E. coli. Antibodies are offered to a range of fusion protein tags, including GST, His, Omni, beta-Gal, S-probe, MBP-probe, Thio-probe, Luciferase, Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP) and the novel CruzTag series of fusion protein tags. Fields and coworkers have developed a unique two-hybrid system using GAL4 fusions in yeast to identify specific protein-protein interactions. The more recently developed Lex A system is a modificaiton of the original Fields system. Antibodies are offered to several fusion protein tags that are used in yeast two-hybrid vectors, including GAL4-TA, GAL4(DBD), Lex A, B42 and VP16.
|
 |
| PRODUCT NAME |
CATALOG # |
ISOTYPE |
EPITOPE |
APPLICATIONS |
SPECIES |
|
| B42 (C-20) |
sc-8607 |
goat IgG |
C-terminal |
WB, IP, IF |
|
| B42 (Q-19) |
sc-8606 |
goat IgG |
internal |
WB, IP, IF |
|
| β-Gal (C-20) |
sc-19119 |
goat IgG |
internal (h) |
WB, IF |
m, r, h |
| β-Gal (H-80) |
sc-20161 |
rabbit IgG |
496-575 (h) |
WB, IP, IF |
human |
| CBP Tag (W-15) |
sc-32998 |
goat IgG |
within the calmodulin binding peptide region (h) |
WB, IP, IF |
|
| CBP Tag (K-24) |
sc-33000 |
goat IgG |
within the calmodulin binding peptide (CBP) region (h)
|
WB, IP, IF |
|
| DsRed (C-20) |
sc-33354 |
goat IgG |
C-terminus |
WB, IF |
|
| DsRed (L-18) |
sc-33353 |
goat IgG |
C-terminus |
WB, IF |
|
| CruzTag 41 (C-12) |
sc-8602 |
goat IgG |
|
WB, IP |
|
| CruzTag 22TM (B-12) |
sc-8601 |
goat IgG |
|
WB, IP |
|
| CruzTag 41 (CZ-C) |
sc-8055 |
mouse IgG |
N/A |
WB, IP |
|
| CruzTag 09TM (CZ-A) |
sc-8053 |
mouse IgG2a |
N/A |
WB, IP |
|
| CruzTag 09TM (A-12) |
sc-8600 |
goat IgG |
CruzTag 09 |
WB, IP |
|
| GAL4 (DBD) (N-19) |
sc-729 |
rabbit IgG |
N-terminal |
WB, IP |
|
| GAL4 (DBD) (RK5C1) |
sc-510 |
mouse IgG2a |
FL |
WB, IP |
|
| GAL4 (DBD) |
sc-577 |
rabbit IgG |
1-147 |
WB, IP |
|
| GAL4-TA (768) |
sc-429 |
rabbit IgG |
768-881 |
WB, IP |
|
| GAL4-TA (C-10) |
sc-1663 |
mouse IgG2b |
768-881 |
WB, IP |
|
| GAL4-TA (A-2) |
sc-46680 |
mouse IgG1 |
768-881 |
WB, IP, IF |
|
| GFP (FL) |
sc-8334 |
rabbit IgG |
1-238 |
WB, IP, IF |
|
| GFP (I-16) |
sc-5385 |
goat IgG |
internal |
WB, IF |
|
| GFP (T-19) |
sc-5384 |
goat IgG |
C-terminus (Av) |
WB, IF |
|
| GFP (B-2) |
sc-9996 |
mouse IgG2a |
1-238 |
WB, IP, IF |
|
| GFP (K-14) |
sc-33852 |
goat IgG |
C-terminus (Rm) |
WB, IF |
Renilla Muelleri |
| GFP (K-17) |
sc-33855 |
goat IgG |
C-terminus (Rr) |
WB, IF |
Renilla Reniformis |
| GST (B-14) |
sc-138 |
mouse IgG1 |
N/A |
WB, IP, IF |
|
| GST (Z-5) |
sc-459 |
rabbit IgG |
|
WB, IP, IF, FCM |
|
| GST (K-18) |
sc-34073 |
goat IgG |
internal |
WB, IF |
Schistosoma japonicum |
| GST (110-218) |
sc-33614 |
rabbit IgG |
110-218 |
WB, IP, IF |
Schistosoma japonicum |
| GST (1-109) |
sc-33613 |
rabbit IgG |
1-109 |
WB, IP, IF |
Schistosoma japonicum |
| GST (E-14) |
sc-34072 |
goat IgG |
N-terminus (schistosoma japonicum) |
WB, IF |
Schistosoma japonicum |
| HA-probe (F-7) |
sc-7392 |
mouse IgG2a |
internal |
WB, IP, IF, FCM |
|
| HA-probe (Y-11) |
sc-805 |
rabbit IgG |
internal |
WB, IP, IF |
|
| HcRed (K-18) |
sc-32189 |
goat IgG |
C-terminus (hc) |
WB, IF |
Heteractis crispa |
| HcRed (N-20) |
sc-32188 |
goat IgG |
N-terminus (Heteractis crispa) |
WB, IF |
|
| His-probe (H-15) |
sc-803 |
rabbit IgG |
|
WB, IP, IF |
|
| His-probe (G-18) |
sc-804 |
rabbit IgG |
|
WB, IP |
|
| His-probe (H-3) |
sc-8036 |
mouse IgG1 |
N/A |
WB, IP |
|
| Lex A (D-19) |
sc-1726 |
goat IgG |
DNA binding domain of Lex A (v) |
WB, IP, IF |
|
| Lex A (N-19) |
sc-1725 |
goat IgG |
N-terminus (E. coli) |
WB, IP, IF |
|
| Lex A (2-12) |
sc-7544 |
mouse IgG1 |
N/A |
WB, IP, IF |
|
| Luciferase (251-550) |
sc-32896 |
rabbit IgG |
251-550 |
WB, IP, IF |
Photinus pyralis |
| OctA-Probe (H-6) |
sc-7787 |
mouse IgG1 |
OctA-probe |
WB, IP, IF. IHC(P) |
OctA and FLAG tagged proteins |
| OctA-Probe (D-8) |
sc-807 |
rabbit IgG |
OctA-probe |
WB, IP, IF. IHC(P) |
OctA and FLAG tagged proteins |
| Omni-probe (D-8) |
sc-7270 |
mouse IgG2a |
WB, IP |
n/a |
|
| Omni-probe (M-21) |
sc-499 |
rabbit IgG |
|
WB, IP |
|
| RrGFP (1-233) |
sc-33150 |
rabbit IgG |
FL |
WB, IP, IF |
Renilla reniformis |
| S-probe (K-14) |
sc-802 |
rabbit IgG |
N/A |
WB |
|
| VP5 (6F10) |
sc-13525 |
mouse IgG1 |
862-880 |
WB, IP, IF |
|
| VP16 (V-20) |
sc-1728 |
goat IgG |
N/A |
WB, IP, IF |
|
| VP16 (1-21) |
sc-7545 |
mouse IgG1 |
456-490 |
WB, IP, IF |
|
| VP16 (14-5) |
sc-7546 |
mouse IgG2a |
N-terminal |
WB, IP, IF |
|
| VP16 (G-18) |
sc-34069 |
goat IgG |
internal |
WB, IF |
|
| VP16 (H-15) |
sc-34070 |
goat IgG |
internal |
WB, IF |
|
| YFP (V-12) |
sc-33262 |
goat IgG |
C-terminus (Vf) |
WB, IF |
Vibrio fischeri |
| YFP (F-20) |
sc-33261 |
goat IgG |
N-terminus (Vibrio fischeri) |
WB, IF |
Vibrio fischeri |
|
