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Plasmid vectors are commonly used for the expression of coding regions of eukaryotic genes in E. coli. Such expression vectors often encode hybrid fusion proteins containing a eukaryotic specified protein fused on a tag protein. Antibodies specific for such fusion protein tags are useful for monitoring protein expression both in plaques and on Western immunoblots as well as for immunoaffinity purification of proteins expressed in E. coli. Antibodies are offered to a range of fusion protein tags, including GST, His, Omni, beta-Gal, S-probe, MBP-probe, Thio-probe, Luciferase, Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP) and the novel CruzTag series of fusion protein tags. Fields and coworkers have developed a unique two-hybrid system using GAL4 fusions in yeast to identify specific protein-protein interactions. The more recently developed Lex A system is a modificaiton of the original Fields system. Antibodies are offered to several fusion protein tags that are used in yeast two-hybrid vectors, including GAL4-TA, GAL4(DBD), Lex A, B42 and VP16.
PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS SPECIES
 B42 (C-20) sc-8607 goat IgG C-terminal WB, IP, IF
 B42 (Q-19) sc-8606 goat IgG internal WB, IP, IF
 β-Gal (C-20) sc-19119 goat IgG internal (h) WB, IF m, r, h
 β-Gal (H-80) sc-20161 rabbit IgG 496-575 (h) WB, IP, IF human
 CBP Tag (W-15) sc-32998 goat IgG within the calmodulin binding peptide region (h) WB, IP, IF
 CBP Tag (K-24) sc-33000 goat IgG within the calmodulin binding peptide (CBP) region (h) WB, IP, IF
 DsRed (C-20) sc-33354 goat IgG C-terminus WB, IF
 DsRed (L-18) sc-33353 goat IgG C-terminus WB, IF
 CruzTag 41 (C-12) sc-8602 goat IgG WB, IP
 CruzTag 22TM (B-12) sc-8601 goat IgG WB, IP
 CruzTag 41 (CZ-C) sc-8055 mouse IgG N/A WB, IP
 CruzTag 09TM (CZ-A) sc-8053 mouse IgG2a N/A WB, IP
 CruzTag 09TM (A-12) sc-8600 goat IgG CruzTag 09 WB, IP
 GAL4 (DBD) (N-19) sc-729 rabbit IgG N-terminal WB, IP
 GAL4 (DBD) (RK5C1) sc-510 mouse IgG2a FL WB, IP
 GAL4 (DBD) sc-577 rabbit IgG 1-147 WB, IP
 GAL4-TA (768) sc-429 rabbit IgG 768-881 WB, IP
 GAL4-TA (C-10) sc-1663 mouse IgG2b 768-881 WB, IP
 GAL4-TA (A-2) sc-46680 mouse IgG1 768-881 WB, IP, IF
 GFP (FL) sc-8334 rabbit IgG 1-238 WB, IP, IF
 GFP (I-16) sc-5385 goat IgG internal WB, IF
 GFP (T-19) sc-5384 goat IgG C-terminus (Av) WB, IF
 GFP (B-2) sc-9996 mouse IgG2a 1-238 WB, IP, IF
 GFP (K-14) sc-33852 goat IgG C-terminus (Rm) WB, IF Renilla Muelleri
 GFP (K-17) sc-33855 goat IgG C-terminus (Rr) WB, IF Renilla Reniformis
 GST (B-14) sc-138 mouse IgG1 N/A WB, IP, IF
 GST (Z-5) sc-459 rabbit IgG WB, IP, IF, FCM
 GST (K-18) sc-34073 goat IgG internal WB, IF Schistosoma japonicum
 GST (110-218) sc-33614 rabbit IgG 110-218 WB, IP, IF Schistosoma japonicum
 GST (1-109) sc-33613 rabbit IgG 1-109 WB, IP, IF Schistosoma japonicum
 GST (E-14) sc-34072 goat IgG N-terminus (schistosoma japonicum) WB, IF Schistosoma japonicum
 HA-probe (F-7) sc-7392 mouse IgG2a internal WB, IP, IF, FCM
 HA-probe (Y-11) sc-805 rabbit IgG internal WB, IP, IF
 HcRed (K-18) sc-32189 goat IgG C-terminus (hc) WB, IF Heteractis crispa
 HcRed (N-20) sc-32188 goat IgG N-terminus (Heteractis crispa) WB, IF
 His-probe (H-15) sc-803 rabbit IgG WB, IP, IF
 His-probe (G-18) sc-804 rabbit IgG WB, IP
 His-probe (H-3) sc-8036 mouse IgG1 N/A WB, IP
 Lex A (D-19) sc-1726 goat IgG DNA binding domain of Lex A (v) WB, IP, IF
 Lex A (N-19) sc-1725 goat IgG N-terminus (E. coli) WB, IP, IF
 Lex A (2-12) sc-7544 mouse IgG1 N/A WB, IP, IF
 Luciferase (251-550) sc-32896 rabbit IgG 251-550 WB, IP, IF Photinus pyralis
 OctA-Probe (H-6) sc-7787 mouse IgG1 OctA-probe WB, IP, IF. IHC(P) OctA and FLAG tagged proteins
 OctA-Probe (D-8) sc-807 rabbit IgG OctA-probe WB, IP, IF. IHC(P) OctA and FLAG tagged proteins
 Omni-probe (D-8) sc-7270 mouse IgG2a WB, IP n/a
 Omni-probe (M-21) sc-499 rabbit IgG WB, IP
 RrGFP (1-233) sc-33150 rabbit IgG FL WB, IP, IF Renilla reniformis
 S-probe (K-14) sc-802 rabbit IgG N/A WB
 VP5 (6F10) sc-13525 mouse IgG1 862-880 WB, IP, IF
 VP16 (V-20) sc-1728 goat IgG N/A WB, IP, IF
 VP16 (1-21) sc-7545 mouse IgG1 456-490 WB, IP, IF
 VP16 (14-5) sc-7546 mouse IgG2a N-terminal WB, IP, IF
 VP16 (G-18) sc-34069 goat IgG internal WB, IF
 VP16 (H-15) sc-34070 goat IgG internal WB, IF
 YFP (V-12) sc-33262 goat IgG C-terminus (Vf) WB, IF Vibrio fischeri
 YFP (F-20) sc-33261 goat IgG N-terminus (Vibrio fischeri) WB, IF Vibrio fischeri